1OPG

OPG2 FAB FRAGMENT

1OPG の概要

• エントリーDOI: 10.2210/pdb1opg/pdb
• 分子名称: OPG2 FAB (LIGHT CHAIN), OPG2 FAB (HEAVY CHAIN) (3 entities in total)
• 機能のキーワード: cell adhesion molecule, immunoglobulin
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Isoform Secreted: Secreted: P01868
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48058.28

構造登録者

Kodandapani, R.,Veerapandian, B.,Ely, K.R. (登録日: 1995-04-28, 公開日: 1995-07-31, 最終更新日: 2024-11-20)

主引用文献

Kodandapani, R.,Veerapandian, B.,Kunicki, T.J.,Ely, K.R.
Crystal structure of the OPG2 Fab. An antireceptor antibody that mimics an RGD cell adhesion site.
J.Biol.Chem., 270:2268-2273, 1995

PubMed Abstract: Cell surface receptors called integrins mediate diverse cell adhesion phenomena through recognition of the sequence arginine-glycine-aspartic acid (RGD) present in proteins such as fibronectin and fibrinogen. Platelet aggregation in hemostasis is mediated by the binding of fibrinogen to the gpIIb/IIIa integrin. The OPG2 antibody binds the gpIIb/IIIa receptor and acts as a ligand mimic due to the presence of an arginine-tyrosine-aspartic acid (RYD) sequence in the CDR3 loop of the heavy chain. The RYD loop and side chains are ordered in the 2.0-A resolution crystal structure of the Fab fragment from this antireceptor antibody. Moreover, the RYD loop assumes two clearly defined conformations that may correspond to the orientations of the loop in the free state or bound to integrin. This molecule will serve as a tool for understanding protein-integrin recognition in platelet aggregation and other RGD-mediated cell adhesion interactions.

PubMed: 7836460
DOI: 10.1074/jbc.270.5.2268

実験手法

X-RAY DIFFRACTION (2 Å)