1OM2

SOLUTION NMR STRUCTURE OF THE MITOCHONDRIAL PROTEIN IMPORT RECEPTOR TOM20 FROM RAT IN A COMPLEX WITH A PRESEQUENCE PEPTIDE DERIVED FROM RAT ALDEHYDE DEHYDROGENASE (ALDH)

1OM2 の概要

• エントリーDOI: 10.2210/pdb1om2/pdb
• NMR情報: BMRB: 4496
• 分子名称: PROTEIN (MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20), PROTEIN (MITOCHONDRIAL ALDEHYDE DEHYDROGENASE) (2 entities in total)
• 機能のキーワード: mitochondrial protein import across outer membrane, receptor for presequences, mitochondrial targeting signal, presequence peptide, receptor-oxidoreductase complex complex, receptor/oxidoreductase complex
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Mitochondrion outer membrane; Single-pass membrane protein (Potential): Q62760; Mitochondrion matrix: P11884
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11697.36

構造登録者

Abe, Y.,Shodai, T.,Muto, T.,Mihara, K.,Torii, H.,Nishikawa, S.,Endo, T.,Kohda, D. (登録日: 1999-04-23, 公開日: 2000-02-02, 最終更新日: 2023-12-27)

主引用文献

Abe, Y.,Shodai, T.,Muto, T.,Mihara, K.,Torii, H.,Nishikawa, S.,Endo, T.,Kohda, D.
Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20.
Cell(Cambridge,Mass.), 100:551-560, 2000

PubMed Abstract: Most mitochondrial proteins are synthesized in the cytosol as precursor proteins with a cleavable N-terminal presequence and are imported into mitochondria. We report here the NMR structure of a general import receptor, rat Tom20, in a complex with a presequence peptide derived from rat aldehyde dehydrogenase. The cytosolic domain of Tom20 forms an all alpha-helical structure with a groove to accommodate the presequence peptide. The bound presequence forms an amphiphilic helical structure with hydrophobic leucines aligned on one side to interact with a hydrophobic patch in the Tom20 groove. Although the positive charges of the presequence are essential for import ability, presequence binding to Tom20 is mediated mainly by hydrophobic rather than ionic interactions.

PubMed: 10721992
DOI: 10.1016/S0092-8674(00)80691-1

実験手法

SOLUTION NMR