1OLD

NMR STRUCTURE OF 24-MER DEOXYRIBONUCLEIC ACID, 7 STRUCTURES

1OLD の概要

• エントリーDOI: 10.2210/pdb1old/pdb
• 分子名称: DNA (GATCGAAACGTAGCGCCTTCGATC), ARGININEAMIDE (2 entities in total)
• 機能のキーワード: deoxyribonucleic acid, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7528.98

構造登録者

Lin, C.H.,Patel, D.J. (登録日: 1996-10-31, 公開日: 1997-04-21, 最終更新日: 2024-05-22)

主引用文献

Lin, C.H.,Patel, D.J.
Encapsulating an amino acid in a DNA fold.
Nat.Struct.Biol., 3:1046-1050, 1996

PubMed Abstract: Here we present the first solution structure of a ligand-DNA aptamer complex. Our NMR-molecular dynamics structural studies of the interaction between argininamide and a DNA stem-loop complex establishes that the hairpin loop DNA binding site undergoes an adaptive conformational transition on complex formation. The tip of the DNA loop folds down towards the stem and sandwiches the bound argininamide between reversed Hoogsteen A.C and Watson-Crick G.C base pairs. The argininamide is encapsulated within the structured DNA loop and is stabilized by an intricate set of intermolecular hydrogen bonds and stacking interactions. The structure of the complex lays out the molecular principles defining both the architecture of the internal cavity and the recognition elements that could contribute to ligand discrimination.

PubMed: 8946859
DOI: 10.1038/nsb1296-1046

実験手法

SOLUTION NMR