1OH6

THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH

1OH6 の概要

• エントリーDOI: 10.2210/pdb1oh6/pdb
• 関連するPDBエントリー: 1E3M 1NG9 1OH5 1OH7 1OH8
• 分子名称: DNA MISMATCH REPAIR PROTEIN MUTS, 5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*AP*GP *CP*AP*CP*CP*AP*GP*TP*GP*TP*CP*AP*GP*CP*GP*TP* CP*CP*TP*AP*T)-3', 5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP *GP*AP*CP*AP*CP*TP*GP*GP*TP*GP*CP*AP*TP*GP*GP* CP*AP*GP*CP*T)-3', ... (6 entities in total)
• 機能のキーワード: dna binding, mismatch recognition
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 198118.11

構造登録者

Natrajan, G.,Lamers, M.H.,Enzlin, J.H.,Winterwerp, H.H.K.,Perrakis, A.,Sixma, T.K. (登録日: 2003-05-23, 公開日: 2003-08-08, 最終更新日: 2023-12-13)

主引用文献

Natrajan, G.,Lamers, M.H.,Enzlin, J.H.,Winterwerp, H.H.K.,Perrakis, A.,Sixma, T.K.
Structures of E. Coli DNA Mismatch Repair Enzyme Muts in Complex with Different Mismatches: A Common Recognition Mode for Diverse Substrates
Nucleic Acids Res., 31:4814-, 2003

PubMed Abstract: We have refined a series of isomorphous crystal structures of the Escherichia coli DNA mismatch repair enzyme MutS in complex with G:T, A:A, C:A and G:G mismatches and also with a single unpaired thymidine. In all these structures, the DNA is kinked by approximately 60 degrees upon protein binding. Two residues widely conserved in the MutS family are involved in mismatch recognition. The phenylalanine, Phe 36, is seen stacking on one of the mismatched bases. The same base is also seen forming a hydrogen bond to the glutamate Glu 38. This hydrogen bond involves the N7 if the base stacking on Phe 36 is a purine and the N3 if it is a pyrimidine (thymine). Thus, MutS uses a common binding mode to recognize a wide range of mismatches.

PubMed: 12907723
DOI: 10.1093/NAR/GKG677

実験手法

X-RAY DIFFRACTION (2.4 Å)