1OGQ

The crystal structure of PGIP (polygalacturonase inhibiting protein), a leucine rich repeat protein involved in plant defense

1OGQ の概要

• エントリーDOI: 10.2210/pdb1ogq/pdb
• 分子名称: POLYGALACTURONASE INHIBITING PROTEIN, 2-acetamido-2-deoxy-beta-D-glucopyranose, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: polygalacturonase inhibiting protein, inhibitor
• 由来する生物種: PHASEOLUS VULGARIS (KIDNEY BEAN)
• 細胞内の位置: Secreted, cell wall: P58822
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34966.72

構造登録者

Di Matteo, A.,Federici, L.,Mattei, B.,Salvi, G.,Johnson, K.A.,Savino, C.,De Lorenzo, G.,Tsernoglou, D.,Cervone, F. (登録日: 2003-05-08, 公開日: 2003-07-24, 最終更新日: 2024-10-23)

主引用文献

Di Matteo, A.,Federici, L.,Mattei, B.,Salvi, G.,Johnson, K.A.,Savino, C.,De Lorenzo, G.,Tsernoglou, D.,Cervone, F.
The Crystal Structure of Polygalacturonase-Inhibiting Protein (Pgip), a Leucine-Rich Repeat Protein Involved in Plant Defense
Proc.Natl.Acad.Sci.USA, 100:10124-, 2003

PubMed Abstract: Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins that protect plants from fungal invasion. They interact with endopolygalacturonases secreted by phytopathogenic fungi, inhibit their enzymatic activity, and favor the accumulation of oligogalacturonides, which activate plant defense responses. PGIPs are members of the leucine-rich repeat (LRR) protein family that in plants play crucial roles in development, defense against pathogens, and recognition of beneficial microbes. Here we report the crystal structure at 1.7-A resolution of a PGIP from Phaseolus vulgaris. The structure is characterized by the presence of two beta-sheets instead of the single one originally predicted by modeling studies. The structure also reveals a negatively charged surface on the LRR concave face, likely involved in binding polygalacturonases. The structural information on PGIP provides a basis for designing more efficient inhibitors for plant protection.

PubMed: 12904578
DOI: 10.1073/PNAS.1733690100

実験手法

X-RAY DIFFRACTION (1.7 Å)