1OGB

Chitinase b from Serratia marcescens mutant D142N

1OGB の概要

• エントリーDOI: 10.2210/pdb1ogb/pdb
• 関連するPDBエントリー: 1H0G 1H0I 1O6I 1OGG
• 分子名称: CHITINASE B, GLYCEROL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, chitin degradation, glycoside hydrolase
• 由来する生物種: SERRATIA MARCESCENS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113824.66

構造登録者

Vaaje-Kolstad, G.,Houston, D.R.,Rao, F.V.,Peter, M.G.,Synstad, B.,van Aalten, D.M.F.,Eijsink, V.G.H. (登録日: 2003-04-29, 公開日: 2004-04-27, 最終更新日: 2024-10-16)

主引用文献

Vaaje-Kolstad, G.,Houston, D.R.,Rao, F.V.,Peter, M.G.,Synstad, B.,van Aalten, D.M.F.,Eijsink, V.G.H.
Structure of the D142N Mutant of the Family 18 Chitinase Chib from Serratia Marcescens and its Complex with Allosamidin
Biochim.Biophys.Acta, 1696:103-111, 2004

PubMed Abstract: Catalysis by ChiB, a family 18 chitinase from Serratia marcescens, involves a conformational change of Asp142 which is part of a characteristic D(140)XD(142)XE(144) sequence motif. In the free enzyme Asp142 points towards Asp140, whereas it rotates towards the catalytic acid, Glu144, upon ligand binding. Mutation of Asp142 to Asn reduced k(cat) and affinity for allosamidin, a competitive inhibitor. The X-ray structure of the D142N mutant showed that Asn142 points towards Glu144 in the absence of a ligand. The active site also showed other structural adjustments (Tyr10, Ser93) that had previously been observed in the wild-type enzyme upon substrate binding. The X-ray structure of a complex of D142N with allosamidin, a pseudotrisaccharide competitive inhibitor, was essentially identical to that of the wild-type enzyme in complex with the same compound. Thus, the reduced allosamidin affinity in the mutant is not caused by structural changes but solely by the loss of electrostatic interactions with Asp142. The importance of electrostatics was further confirmed by the pH dependence of catalysis and allosamidin inhibition. The pH-dependent apparent affinities for allosamidin were not correlated with k(cat), indicating that it is probably better to view the inhibitor as a mimic of the oxazolinium ion reaction intermediate than as a transition state analogue.

PubMed: 14726210
DOI: 10.1016/j.bbapap.2003.09.014

実験手法

X-RAY DIFFRACTION (1.85 Å)