1OFX

CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION

1OFX の概要

• エントリーDOI: 10.2210/pdb1ofx/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*TP*AP*TP*AP*CP*GP*C)-3'), DNA/RNA (5'-R(*GP*CP*GP*)-D(*TP*AP*TP*AP*CP*CP*C)-3'), SPERMINE, ... (4 entities in total)
• 機能のキーワード: a-dna/rna, double helix, dna-rna hybrid
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6340.35

構造登録者

Egli, M.,Usman, N.,Zhang, S.,Rich, A. (登録日: 1991-10-17, 公開日: 1993-04-15, 最終更新日: 2024-02-14)

主引用文献

Egli, M.,Usman, N.,Zhang, S.G.,Rich, A.
Crystal structure of an Okazaki fragment at 2-A resolution.
Proc.Natl.Acad.Sci.USA, 89:534-538, 1992

PubMed Abstract: In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.

PubMed: 1370582
DOI: 10.1073/pnas.89.2.534

実験手法

X-RAY DIFFRACTION (2 Å)