1OFV

FLAVODOXIN FROM ANACYSTIS NIDULANS: REFINEMENT OF TWO FORMS OF THE OXIDIZED PROTEIN

1OFV の概要

• エントリーDOI: 10.2210/pdb1ofv/pdb
• 分子名称: FLAVODOXIN, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Synechococcus elongatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19112.64

構造登録者

Smith, W.W.,Pattridge, K.A.,Luschinsky, C.L.,Ludwig, M.L. (登録日: 1992-06-22, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Drennan, C.L.,Pattridge, K.A.,Weber, C.H.,Metzger, A.L.,Hoover, D.M.,Ludwig, M.L.
Refined structures of oxidized flavodoxin from Anacystis nidulans.
J.Mol.Biol., 294:711-724, 1999

PubMed Abstract: Flavodoxin from Anacystis nidulans (Synechococcus PCC 7942) was the first member of the flavodoxin family to be characterized, and is the structural prototype for the "long-chain" flavodoxins that have molecular masses of approximately 20 kDa. Crystal structure analyses and refinements of three orthorhombic forms of oxidized A. nidulans flavodoxin are reported, and salient features of the fold and the FMN binding site are compared with other flavodoxins. The structure of form I (wild-type: P212121, a=57.08 A, b=69.24 A, c=45.55 A), determined initially by multiple isomorphous replacement, has been refined to R=0.183 and R(free)=0.211 for data from 10.0 to 1.7 A resolution. Structures of form II (wild-type: P212121, a=60.05 A, b=65.85 A, c=51.36 A) and form III (Asn58Gly: P212121, a=51.30 A, b=59.15 A, c=94.44 A) have been determined by molecular replacement and refined versus data to 2.0 A and 1.85 A, respectively; the R values for forms II and III are 0.147 and 0.150. Changes in the molecular contacts that produce the alternative packings in these crystalline forms are analyzed. Deletion of the Asn side-chain in the mutant Asn58Gly removes an intermolecular stacking interaction and allows the alternative packing found in form III crystals. The functionally important 50's loop of the FMN binding site is less restrained by intermolecular contacts in these crystals but maintains the same conformation as in oxidized wild type protein. The structures reported here provide the starting point for structure-function studies of the reduced states and of mutants, described in the accompanying paper.

PubMed: 10610791
DOI: 10.1006/jmbi.1999.3151

実験手法

X-RAY DIFFRACTION (1.7 Å)