1OF4

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27

1OF4 の概要

• エントリーDOI: 10.2210/pdb1of4/pdb
• 関連するPDBエントリー: 1OF3
• 分子名称: BETA-MANNOSIDASE, beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: hydrolase/carbohydrate binding, mannan binding, carbohydrate binding module, polysaccharide degradation, hydrolase, hydrolase-carbohydrate binding complex
• 由来する生物種: THERMOTOGA MARITIMA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21542.00

構造登録者

Boraston, A.B.,Revett, T.J.,Boraston, C.M.,Nurizzo, D.,Davies, G.J. (登録日: 2003-04-07, 公開日: 2003-04-17, 最終更新日: 2024-05-08)

主引用文献

Boraston, A.B.,Revett, T.J.,Boraston, C.M.,Nurizzo, D.,Davies, G.J.
Structural and Thermodynamic Dissection of Specific Mannan Recognition by a Carbohydrate Binding Module, Tmcbm27
Structure, 11:665-, 2003

PubMed Abstract: The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5) constitute a carbohydrate binding module (CBM) that has been classified into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6 A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for mannans. In particular, the latter complex, which is the first structure of a CBM in complex with a branched plant cell wall polysaccharide, illustrates how the architecture of the binding site can influence the recognition of naturally substituted polysaccharides.

PubMed: 12791255
DOI: 10.1016/S0969-2126(03)00100-X

実験手法

X-RAY DIFFRACTION (1.6 Å)