1OEE

YodA from Escherichia coli crystallised with cadmium ions

1OEE の概要

• エントリーDOI: 10.2210/pdb1oee/pdb
• 関連するPDBエントリー: 1OEJ 1OEK
• 分子名称: HYPOTHETICAL PROTEIN YODA, CADMIUM ION (3 entities in total)
• 機能のキーワード: stress protein/lipocalin, stress protein, lipocalin, yoda, cadmium, stress protein-lipocalin complex
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cytoplasm: P76344
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22823.52

構造登録者

David, G.,Blondeau, K.,Renouard, M.,Penel, S.,Lewit-Bentley, A. (登録日: 2003-03-27, 公開日: 2003-08-15, 最終更新日: 2024-11-20)

主引用文献

David, G.,Blondeau, K.,Schiltz, M.,Penel, S.,Lewit-Bentley, A.
Yoda from Escherichia Coli is a Metal-Binding, Lipocalin-Like Protein
J.Biol.Chem., 278:43728-, 2003

PubMed Abstract: We have determined the crystal structure of YodA, an Escherichia coli protein of unknown function. YodA had been identified under conditions of cadmium stress, and we confirm that it binds metals such as cadmium and zinc. We have also found nickel bound in one of the crystal forms. YodA is composed of two domains: a main lipocalin/calycin-like domain and a helical domain. The principal metal-binding site lies on one side of the calycin domain, thus making YodA the first metal-binding lipocalin known. Our experiments suggest that YodA expression may be part of a more general stress response. From sequence analogy with the C-terminal domain of a metal-binding receptor of a member of bacterial ATP-binding cassette transporters, we propose a three-dimensional model for this receptor and suggest that YodA may have a receptor-type partner in E. coli.

PubMed: 12909634
DOI: 10.1074/JBC.M304484200

実験手法

X-RAY DIFFRACTION (2.1 Å)