1OBW

STRUCTURE OF INORGANIC PYROPHOSPHATASE

1OBW の概要

• エントリーDOI: 10.2210/pdb1obw/pdb
• 分子名称: INORGANIC PYROPHOSPHATASE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: hydrolase, magnesium, metal binding
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A7A9
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 58925.97

構造登録者

Oganessyan, V.Yu.,Harutyunyan, E.H.,Avaeva, S.M.,Oganessyan, N.N.,Mather, T.,Huber, R. (登録日: 1996-10-09, 公開日: 1997-09-04, 最終更新日: 2024-04-03)

主引用文献

Harutyunyan, E.H.,Oganessyan, V.Y.,Oganessyan, N.N.,Avaeva, S.M.,Nazarova, T.I.,Vorobyeva, N.N.,Kurilova, S.A.,Huber, R.,Mather, T.
Crystal structure of holo inorganic pyrophosphatase from Escherichia coli at 1.9 A resolution. Mechanism of hydrolysis.
Biochemistry, 36:7754-7760, 1997

PubMed Abstract: Crystalline holo inorganic pyrophosphatase from Escherichia coli was grown in the presence of 250 mM MgCl2. The crystal structure has been solved by Patterson search techniques and refined to an R-factor of 17.6% at 1.9 A resolution. The upper estimate of the root-mean-square error in atomic positions is 0.26 A. These crystals belong to space group P3(2)21 with unit cell dimensions a = b = 110.27 A and c = 78.17 A. The asymmetric unit contains a trimer of subunits, i.e., half of the hexameric molecule. In the central cavity of the enzyme molecule, three Mg2+ ions, each shared by two subunits of the hexamer, are found. In the active sites of two crystallographically independent subunits, two Mg2+ ions are bound. The second active site Mg2+ ion is missing in the third subunit. A mechanism of catalysis is proposed whereby a water molecule activated by a Mg2+ ion and Tyr 55 play essential roles.

PubMed: 9201917
DOI: 10.1021/bi962637u

実験手法

X-RAY DIFFRACTION (1.9 Å)