1OAZ

IgE Fv SPE7 complexed with a recombinant thioredoxin

1OAZ の概要

• エントリーDOI: 10.2210/pdb1oaz/pdb
• 関連するPDBエントリー: 1F6M 1KEB 1M7T 1SRX 1T7P 1THO 1TXX 1XOA 1XOB 2TIR 2TRX
• 分子名称: THIOREDOXIN 1, IMMUNOGLOBULIN E, ... (4 entities in total)
• 機能のキーワード: immune system, antibody-complex, antibody, allergy, ige, conformational diversity, multispecficity, redox-active center, electron transport
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 77368.90

構造登録者

James, L.C.,Roversi, P.,Tawfik, D. (登録日: 2003-01-21, 公開日: 2004-01-15, 最終更新日: 2026-03-04)

主引用文献

James, L.C.,Roversi, P.,Tawfik, D.
Antibody Multispecificity Mediated by Conformational Diversity
Science, 299:1362-, 2003

PubMed Abstract: A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.

PubMed: 12610298
DOI: 10.1126/SCIENCE.1079731

実験手法

X-RAY DIFFRACTION (2.78 Å)