1O8R

Solution structure of human proguanylin

1O8R の概要

• エントリーDOI: 10.2210/pdb1o8r/pdb
• 関連するPDBエントリー: 1GNA 1GNB
• 分子名称: GUANYLIN (1 entity in total)
• 機能のキーワード: prohormone, proguanylin, guanylin, gap-i, precursor, prosequence
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted: Q02747
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10351.79

構造登録者

Lauber, T.,Rosch, P.,Marx, U.C. (登録日: 2002-11-28, 公開日: 2003-04-24, 最終更新日: 2024-10-09)

主引用文献

Lauber, T.,Neudecker, P.,Roesch, P.,Marx, U.C.
Solution Structure of Human Proguanylin: The Role of a Hormone Prosequence
J.Biol.Chem., 278:24118-, 2003

PubMed Abstract: The endogenous ligand of guanylyl cyclase C, guanylin, is produced as the 94-amino-acid prohormone proguanylin, with the hormone guanylin located at the COOH terminus of the prohormone. The solution structure of proguanylin adopts a new protein fold and consists of a three-helix bundle, a small three-stranded beta-sheet of two NH2-terminal strands and one COOH-terminal strand, and an unstructured linker region. The sequence corresponding to guanylin is fixed in its bioactive topology and is involved in interactions with the NH2-terminal beta-hairpin: the hormone region (residues 80-94) partly wraps around the first 4 NH2-terminal residues that thereby shield parts of the hormone surface. These interactions provide an explanation for the negligible bioactivity of the prohormone as well as the important role of the NH2-terminal residues in the disulfide-coupled folding of proguanylin. Since the ligand binding region of guanylyl cyclase C is predicted to be located around an exposed beta-strand, the intramolecular interactions observed between guanylin and its prosequence may be comparable with the guanylin/receptor interaction.

PubMed: 12707255
DOI: 10.1074/JBC.M300370200

実験手法

SOLUTION NMR