1O8J

Pectate Lyase C from Erwinia Chrysanthemi at pH 4.5 with 30mM CA2+

1O8J の概要

• エントリーDOI: 10.2210/pdb1o8j/pdb
• 関連するPDBエントリー: 1AIR 1O88 1O8D 1O8E 1O8F 1O8G 1O8H 1O8I 1O8K 1O8L 1O8M 1PLU 2PEC
• 分子名称: PECTATE LYASE C, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrolase, pectate lyase cleavage, calcium binding, parallel beta-helix
• 由来する生物種: ERWINIA CHRYSANTHEMI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37773.68

構造登録者

Herron, S.R.,Jurnak, F.A. (登録日: 2002-11-27, 公開日: 2003-01-30, 最終更新日: 2023-12-13)

主引用文献

Herron, S.R.,Scavetta, R.,Garrett, M.,Legner, M.,Jurnak, F.A.
Characterization and Implications of Ca2+ Binding to Pectate Lyase C
J.Biol.Chem., 278:12271-12277, 2003

PubMed Abstract: Ca(2+) is essential for in vitro activity of Erwinia chrysanthemi pectate lyase C (PelC). Crystallographic analyses of 11 PelC-Ca(2+) complexes, formed at pH 4.5, 9.5, and 11.2 under varying Ca(2+) concentrations, have been solved and refined at a resolution of 2.2 A. The Ca(2+) site represents a new motif for Ca(2+), consisting primarily of beta-turns and beta-strands. The principal differences between PelC and the PelC-Ca(2+) structures at all pH values are the side-chain conformations of Asp-129 and Glu-166 as well as the occupancies of four water molecules. According to calculations of pK(a) values, the presence of Ca(2+) and associated structural changes lower the pK(a) of Arg-218, the amino acid responsible for proton abstraction during catalysis. The Ca(2+) affinity for PelC is weak, as the K(d) was estimated to be 0.132 (+/-0.004) mm at pH 9.5, 1.09 (+/-0.29) mm at pH 11.2, and 5.84 (+/-0.41) mm at pH 4.5 from x-ray diffraction studies and 0.133 (+/-0.045) mm at pH 9.5 from intrinsic tryptophan fluorescence measurements. Given the pH dependence of Ca(2+) affinity, PelC activity at pH 4.5 has been reexamined. At saturating Ca(2+) concentrations, PelC activity increases 10-fold at pH 4.5 but is less than 1% of maximal activity at pH 9.5. Taken together, the studies suggest that the primary Ca(2+) ion in PelC has multiple functions.

PubMed: 12540845
DOI: 10.1074/JBC.M209306200

実験手法

X-RAY DIFFRACTION (2.2 Å)