1O7X

Citrate synthase from Sulfolobus solfataricus

1O7X の概要

• エントリーDOI: 10.2210/pdb1o7x/pdb
• 分子名称: CITRATE SYNTHASE (1 entity in total)
• 機能のキーワード: synthase, lyase, tricarboxylic acid cycle
• 由来する生物種: SULFOLOBUS SOLFATARICUS
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 171224.24

構造登録者

Bell, G.S.,Russell, R.J.M.,Connaris, H.,Hough, D.W.,Danson, M.J.,Taylor, G.L. (登録日: 2002-11-19, 公開日: 2002-12-12, 最終更新日: 2024-05-08)

主引用文献

Bell, G.S.,Russell, R.J.M.,Connaris, H.,Hough, D.W.,Danson, M.J.,Taylor, G.L.
Stepwise Adaptations of Citrate Synthase to Survival at Life'S Extremes. From Psychrophile to Hyperthermophile.
Eur.J.Biochem., 269:6250-, 2002

PubMed Abstract: The crystal structure of citrate synthase from the thermophilic Archaeon Sulfolobus solfataricus (optimum growth temperature = 85 degrees C) has been determined, extending the number of crystal structures of citrate synthase from different organisms to a total of five that span the temperature range over which life exists (from psychrophile to hyperthermophile). Detailed structural analysis has revealed possible molecular mechanisms that determine the different stabilities of the five proteins. The key to these mechanisms is the precise structural location of the additional interactions. As one ascends the temperature ladder, the subunit interface of this dimeric enzyme and loop regions are reinforced by complex electrostatic interactions, and there is a reduced exposure of hydrophobic surface. These observations reveal a progressive pattern of stabilization through multiple additional interactions at solvent exposed, loop and interfacial regions.

PubMed: 12473121
DOI: 10.1046/J.1432-1033.2002.03344.X

実験手法

X-RAY DIFFRACTION (2.7 Å)