1O7S

High resolution structure of Siglec-7

1O7S の概要

• エントリーDOI: 10.2210/pdb1o7s/pdb
• 関連するPDBエントリー: 1O7V
• 分子名称: SIALIC ACID BINDING IG-LIKE LECTIN 7, CYSTEINE, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: siglec, immunoglobulin fold, sialic acid binding protein, lectin, cell adhesion, immune system
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: Q9Y286
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15218.87

構造登録者

Alphey, M.S.,Attrill, H.,Crocker, P.R.,Van Aalten, D.M.F. (登録日: 2002-11-12, 公開日: 2003-03-30, 最終更新日: 2023-12-13)

主引用文献

Alphey, M.S.,Attrill, H.,Crocker, P.R.,Van Aalten, D.M.F.
High Resolution Structures of Siglec-7 - Insights Into Ligand Specificity in the Siglec Family
J.Biol.Chem., 278:3372-, 2003

PubMed Abstract: Sialic acid-binding immunoglobulin-like lectins (Siglecs) recognize sialylated glycoconjugates and play a role in cell-cell recognition. Siglec-7 is expressed on natural killer cells and displays unique ligand binding properties different from other members of the Siglec family. Here we describe the high resolution structures of the N-terminal V-set Ig-like domain of Siglec-7 in two crystal forms, at 1.75 and 1.9 A. The latter crystal form reveals the full structure of this domain and allows us to speculate on the differential ligand binding properties displayed by members of the Siglec family. A fully ordered N-linked glycan is observed, tethered by tight interactions with symmetry-related protein molecules in the crystal. Comparison of the structure with that of sialoadhesin and a model of Siglec-9 shows that the unique preference of Siglec-7 for alpha(2,8)-linked disialic acid is likely to reside in the C-C' loop, which is variable in the Siglec family. In the Siglec-7 structure, the ligand-binding pocket is occupied by a loop of a symmetry-related molecule, mimicking the interactions with sialic acid.

PubMed: 12438315
DOI: 10.1074/JBC.M210602200

実験手法

X-RAY DIFFRACTION (1.75 Å)