1O7G

NAPHTHALENE 1,2-DIOXYGENASE WITH NAPHTHALENE BOUND IN THE ACTIVE SITE.

1O7G の概要

• エントリーDOI: 10.2210/pdb1o7g/pdb
• 関連するPDBエントリー: 1EG9 1NDO 1O7H 1O7M 1O7N 1O7P 1O7W
• 分子名称: NAPHTHALENE 1,2-DIOXYGENASE ALPHA SUBUNIT, NAPHTHALENE 1,2-DIOXYGENASE BETA SUBUNIT, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: oxidoreductase, non-heme iron dioxygenase, enzyme-substrate complex, iron-sulfur, aromatic hydrocarbon catabolism
• 由来する生物種: PSEUDOMONAS PUTIDA; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73653.88

構造登録者

Karlsson, A.,Parales, J.V.,Parales, R.E.,Gibson, D.T.,Eklund, H.,Ramaswamy, S. (登録日: 2002-11-05, 公開日: 2003-02-20, 最終更新日: 2024-11-20)

主引用文献

Karlsson, A.,Parales, J.V.,Parales, R.E.,Gibson, D.T.,Eklund, H.,Ramaswamy, S.
Crystal Structure of Naphthalene Dioxygenase: Side-on Binding of Dioxygen to Iron
Science, 299:1039-, 2003

PubMed Abstract: Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase.

PubMed: 12586937
DOI: 10.1126/SCIENCE.1078020

実験手法

X-RAY DIFFRACTION (1.7 Å)