1O55

MOLECULAR STRUCTURE OF TWO CRYSTAL FORMS OF CYCLIC TRIADENYLIC ACID AT 1 ANGSTROM RESOLUTION

「415D」から置き換えられました

1O55 の概要

• エントリーDOI: 10.2210/pdb1o55/pdb
• 分子名称: DNA (5'-CD(*AP*AP*AP)-3'), COBALT (II) ION (3 entities in total)
• 機能のキーワード: cyclic trinucleotide, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 1848.26

構造登録者

Gao, Y.G.,Robinson, H.,Guan, Y.,Liaw, Y.C.,van Boom, J.H.,van der Marel, G.A.,Wang, A.H. (登録日: 2003-08-20, 公開日: 2003-08-26, 最終更新日: 2023-12-27)

主引用文献

Gao, Y.G.,Robinson, H.,Guan, Y.,Liaw, Y.C.,van Boom, J.H.,van der Marel, G.A.,Wang, A.H.
Molecular structure of two crystal forms of cyclic triadenylic acid at 1A resolution.
J.Biomol.Struct.Dyn., 16:69-76, 1998

PubMed Abstract: The three dimensional structures of cyclic deoxytriadenylic acid, c-d(ApApAp), from two different trigonal crystal forms (space groups P3 and R32) have been determined by x-ray diffraction analysis at 1A resolution. Both structures were solved by direct methods and refined by anisotropic least squares refinement to R-factors of 0.109 and 0.137 for the P3 and R32 forms, respectively. In both crystal forms, each of the two independent c-d(ApApAp) molecules sits on the crystallographic 3-fold axis. All four independent c-d(ApApAp) molecules have similar backbone conformations. The deoxyriboses are in the S-type pucker with pseudorotation angles ranging from 156.7 degrees to 168.6 degrees and the bases have anti glycosyl torsion angles (chi falling in two ranges, one at -104.3 degrees and the other ranging from -141.0 degrees to -143.8 degrees). In the R32 form, a hexahydrated cobalt(II) ion is found to coordinate through bridging water molecules to N1, N3, and N7 atoms of three adjacent adenines and oxygen atoms of phosphates. Comparison with other structures of cyclic oligonucleotides indicates that the sugar adopts N-type pucker in cyclic dinucleotides and S-type pucker in cyclic trinucleotides, regardless whether the sugar is a ribose or a deoxyribose.

PubMed: 9745896

実験手法

X-RAY DIFFRACTION (1.04 Å)