1O06

Crystal structure of the Vps27p Ubiquitin Interacting Motif (UIM)

1O06 の概要

• エントリーDOI: 10.2210/pdb1o06/pdb
• 分子名称: Vacuolar protein sorting-associated protein VPS27, ZINC ION (3 entities in total)
• 機能のキーワード: alpha-helix, coiled-coil, tetramer, transport protein
• 細胞内の位置: Endosome membrane; Peripheral membrane protein; Cytoplasmic side: P40343
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2441.58

構造登録者

Fisher, R.D.,Wang, B.,Alam, S.L.,Higginson, D.S.,Rich, R.,Myszka, D.,Sundquist, W.I.,Hill, C.P. (登録日: 2003-02-20, 公開日: 2003-07-22, 最終更新日: 2024-02-14)

主引用文献

Fisher, R.D.,Wang, B.,Alam, S.L.,Higginson, D.S.,Robinson, H.,Sundquist, W.I.,Hill, C.P.
Structure and ubiquitin binding of the ubiquitin-interacting motif.
J.Biol.Chem., 278:28976-28984, 2003

PubMed Abstract: Ubiquitylation is used to target proteins into a large number of different biological processes including proteasomal degradation, endocytosis, virus budding, and vacuolar protein sorting (Vps). Ubiquitylated proteins are typically recognized using one of several different conserved ubiquitin binding modules. Here, we report the crystal structure and ubiquitin binding properties of one such module, the ubiquitin-interacting motif (UIM). We found that UIM peptides from several proteins involved in endocytosis and vacuolar protein sorting including Hrs, Vps27p, Stam1, and Eps15 bound specifically, but with modest affinity (Kd = 0.1-1 mm), to free ubiquitin. Full affinity ubiquitin binding required the presence of conserved acidic patches at the N and C terminus of the UIM, as well as highly conserved central alanine and serine residues. NMR chemical shift perturbation mapping experiments demonstrated that all of these UIM peptides bind to the I44 surface of ubiquitin. The 1.45 A resolution crystal structure of the second yeast Vps27p UIM (Vps27p-2) revealed that the ubiquitin-interacting motif forms an amphipathic helix. Although Vps27p-2 is monomeric in solution, the motif unexpectedly crystallized as an antiparallel four-helix bundle, and the potential biological implications of UIM oligomerization are therefore discussed.

PubMed: 12750381
DOI: 10.1074/jbc.M302596200

実験手法

X-RAY DIFFRACTION (1.45 Å)