1NZ9

Solution Structure of the N-utilization substance G (NusG) C-terminal (NGC) domain from Thermus thermophilus

1NZ9 の概要

• エントリーDOI: 10.2210/pdb1nz9/pdb
• 関連するPDBエントリー: 1NZ8
• 分子名称: TRANSCRIPTION ANTITERMINATION PROTEIN NUSG (1 entity in total)
• 機能のキーワード: transcription elongation, termination, antitermination, riken structural genomics/proteomics initiative, rsgi, structural genomics, transcription
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6361.22

構造登録者

Reay, P.,Yamasaki, K.,Terada, T.,Kuramitsu, S.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-02-17, 公開日: 2004-04-06, 最終更新日: 2024-05-29)

主引用文献

Reay, P.,Yamasaki, K.,Terada, T.,Kuramitsu, S.,Shirouzu, M.,Yokoyama, S.
Structural and sequence comparisons arising from the solution structure of the transcription elongation factor NusG from Thermus thermophilus
Proteins, 56:40-51, 2004

PubMed Abstract: NusG is an essential bacterial protein modulator of transcriptional elongation and termination events, and interacts directly with RNA polymerase and Rho protein. Found also in Archaea, NusG shows stretches of sequence similarity to the eukaryotic transcription elongation factor Spt5. Herein, the three-dimensional solution structure of the bacterial NusG from Thermus thermophilus, which shows 43% amino acid sequence similarity to the Escherichia coli NusG, is described, and a survey of NusG and Spt5 amino acid sequences is presented. Although there is a clear evolutionary and functional relationship between these proteins, it is evident from the structural, sequence, and biochemical data that their binding specificities to both nucleic acids and other proteins differ.

PubMed: 15162485
DOI: 10.1002/prot.20054

実験手法

SOLUTION NMR