1NY7

COWPEA MOSAIC VIRUS (CPMV)

1NY7 の概要

• エントリーDOI: 10.2210/pdb1ny7/pdb
• 関連するPDBエントリー: 1BMV
• 分子名称: COWPEA MOSAIC VIRUS, SMALL (S) SUBUNIT, COWPEA MOSAIC VIRUS, LARGE (L) SUBUNIT (3 entities in total)
• 機能のキーワード: comovirus, virus, viral coat protein, cowpea mosaic virus (cpmv), icosahedral virus
• 由来する生物種: Cowpea mosaic virus; 詳細
• 細胞内の位置: Movement protein: Host cell junction, host plasmodesma. Large coat protein: Virion : P03599 P03599
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61820.00

構造登録者

Lin, T.,Chen, Z.,Usha, R.,Stauffacher, C.V.,Dai, J.-B.,Schmidt, T.,Johnson, J.E. (登録日: 2003-02-11, 公開日: 2003-03-18, 最終更新日: 2024-10-16)

主引用文献

Lin, T.,Chen, Z.,Usha, R.,Stauffacher, C.V.,Dai, J.-B.,Schmidt, T.,Johnson, J.E.
The Refined Crystal Structure of Cowpea Mosaic Virus at 2.8A Resolution
Virology, 265:20-34, 1999

PubMed Abstract: Comoviruses are a group of plant viruses in the picornavirus superfamily. The type member of comoviruses, cowpea mosaic virus (CPMV), was crystallized in the cubic space group I23, a = 317 A and the hexagonal space group P6(1)22, a = 451 A, c = 1038 A. Structures of three closely similar nucleoprotein particles were determined in the cubic form. The roughly 300-A capsid was similar to the picornavirus capsid displaying a pseudo T = 3 (P = 3) surface lattice. The three beta-sandwich domains adopt two orientations, one with the long axis radial and the other two with the long axes tangential in reference to the capsid sphere. T = 3 viruses display one or the other of these two orientations. The CPMV capsid was permeable to cesium ions, leading to a disturbance of the beta-annulus inside a channel-like structure, suggesting an ion channel. The hexagonal crystal form diffracted X rays to 3 A resolution, despite the large unit cell. The large ( approximately 200 A) solvent channels in the lattice allow exchange of CPMV cognate Fab fragments. As an initial step in the structure determination of the CPMV/Fab complex, the P6(1)22 crystal structure was solved by molecular replacement with the CPMV model determined in the cubic cell.

PubMed: 10603314
DOI: 10.1006/viro.1999.0038

実験手法

X-RAY DIFFRACTION (3 Å)