1NY4

Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.

1NY4 の概要

• エントリーDOI: 10.2210/pdb1ny4/pdb
• NMR情報: BMRB: 5691
• 分子名称: 30S ribosomal protein S28E (1 entity in total)
• 機能のキーワード: jr19, autostructure, ribosomal protein, northeast structural genomics consortium, psi, protein structure initiative, nesg, rna binding protein
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9386.69

構造登録者

Aramini, J.M.,Cort, J.R.,Huang, Y.J.,Xiao, R.,Acton, T.B.,Ho, C.K.,Shih, L.-Y.,Kennedy, M.A.,Montelione, G.T.,Northeast Structural Genomics Consortium (NESG) (登録日: 2003-02-11, 公開日: 2003-09-02, 最終更新日: 2024-05-22)

主引用文献

Aramini, J.M.,Huang, Y.J.,Cort, J.R.,Goldsmith-Fischman, S.,Xiao, R.,Shih, L.-Y.,Ho, C.K.,Liu, J.,Rost, B.,Honig, B.,Kennedy, M.A.,Acton, T.B.,Montelione, G.T.
Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii.
Protein Sci., 12:2823-2830, 2003

PubMed Abstract: We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

PubMed: 14627742
DOI: 10.1110/ps.03359003

実験手法

SOLUTION NMR