1NWV

SOLUTION STRUCTURE OF A FUNCTIONALLY ACTIVE COMPONENT OF DECAY ACCELERATING FACTOR

1NWV の概要

• エントリーDOI: 10.2210/pdb1nwv/pdb
• NMR情報: BMRB: 5506
• 分子名称: Complement decay-accelerating factor (1 entity in total)
• 機能のキーワード: cd55, daf, ccp, complement, biosynthetic protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI- anchor: P08174
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14351.30

構造登録者

Uhrinova, S.,Lin, F.,Ball, G.,Bromek, K.,Uhrin, D.,Medof, M.E.,Barlow, P.N. (登録日: 2003-02-07, 公開日: 2003-04-22, 最終更新日: 2024-10-16)

主引用文献

Uhrinova, S.,Lin, F.,Ball, G.,Bromek, K.,Uhrin, D.,Medof, M.E.,Barlow, P.N.
Solution structure of a functionally active fragment of decay-accelerating factor
Proc.Natl.Acad.Sci.USA, 100:4718-4723, 2003

PubMed Abstract: The second and third modules of human decay accelerating factor (DAF) are necessary and sufficient to accelerate decay of the classical pathway (CP) convertase of complement. No structure of a mammalian protein with decay-accelerating activity has been available to date. We therefore determined the solution structure of DAF modules 2 and 3 (DAF approximately 2,3). Structure-guided analysis of 24 mutants identified likely contact points between DAF and the CP convertase. Three (R96, R69, and a residue in the vicinity of L171) lie on DAF approximately 2,3's concave face. A fourth, consisting of K127 and nearby R100, is on the opposite face. Regions of module 3 remote from the semiflexible 2-3 interface seem not to be involved in binding to the CP convertase. DAF thus seems to occupy a groove on the CP convertase such that both faces of DAF close to the 2-3 junction (including a positively charged region that encircles the protein at this point) interact simultaneously. Alternative pathway convertase interactions with DAF require additional regions of CCP 3 lying away from the 2-3 interface, consistent with the established additional requirement of module 4 for alternative pathway regulation.

PubMed: 12672958
DOI: 10.1073/pnas.0730844100

実験手法

SOLUTION NMR