1NW1

Crystal Structure of Choline Kinase

1NW1 の概要

• エントリーDOI: 10.2210/pdb1nw1/pdb
• 分子名称: Choline kinase (49.2 kD), CALCIUM ION (3 entities in total)
• 機能のキーワード: choline kinase, phospholipid synthesis, protein kinase fold, transferase
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 98682.13

構造登録者

Peisach, D.,Gee, P.,Kent, C.,Xu, Z. (登録日: 2003-02-05, 公開日: 2003-06-10, 最終更新日: 2024-02-14)

主引用文献

Peisach, D.,Gee, P.,Kent, C.,Xu, Z.
The Crystal Structure of Choline Kinase Reveals a Eukaryotic Protein Kinase Fold
Structure, 11:703-713, 2003

PubMed Abstract: Choline kinase catalyzes the ATP-dependent phosphorylation of choline, the first committed step in the CDP-choline pathway for the biosynthesis of phosphatidylcholine. The 2.0 A crystal structure of a choline kinase from C. elegans (CKA-2) reveals that the enzyme is a homodimeric protein with each monomer organized into a two-domain fold. The structure is remarkably similar to those of protein kinases and aminoglycoside phosphotransferases, despite no significant similarity in amino acid sequence. Comparisons to the structures of other kinases suggest that ATP binds to CKA-2 in a pocket formed by highly conserved and catalytically important residues. In addition, a choline binding site is proposed to be near the ATP binding pocket and formed by several structurally flexible loops.

PubMed: 12791258
DOI: 10.1016/S0969-2126(03)00094-7

実験手法

X-RAY DIFFRACTION (2.02 Å)