1NVB

Crystal structure of 3-dehydroquinate synthase (DHQS) in complex with ZN2+ and carbaphosphonate

Summary for 1NVB

• Entry DOI: 10.2210/pdb1nvb/pdb
• Related: 1NR5 1NRX 1NUA 1NVA 1NVD 1NVE 1NVF
• Descriptor: 3-DEHYDROQUINATE SYNTHASE, ZINC ION, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: shikimate pathway, aromatic amino acid biosynthesis, dhqs, closed form, form c, domain movement, cyclase, lyase
• Biological source: Emericella nidulans
• Cellular location: Cytoplasm: P07547
• Total number of polymer chains: 2
• Total formula weight: 87338.57

Authors

Nichols, C.E.,Ren, J.,Lamb, H.K.,Hawkins, A.R.,Stammers, D.K. (deposition date: 2003-02-03, release date: 2003-03-18, Last modification date: 2023-10-25)

Primary citation

Nichols, C.E.,Ren, J.,Lamb, H.K.,Hawkins, A.R.,Stammers, D.K.
Ligand-induced Conformational Changes and a Mechanism for Domain Closure in Aspergillus nidulans Dehydroquinate Synthase
J.MOL.BIOL., 327:129-144, 2003

PubMed Abstract: In order to investigate systematically substrate and cofactor-induced conformational changes in the enzyme dehydroquinate synthase (DHQS), eight structures representing a series of differently liganded states have been determined in a total of six crystal forms. DHQS in the absence of the substrate analogue carbaphosphonate, either unliganded or in the presence of NAD or ADP, is in an open form where a relative rotation of 11-13 degrees between N and C-terminal domains occurs. Analysis of torsion angle difference plots between sets of structures reveals eight rearrangements that appear relevant to domain closure and a further six related to crystal packing. Overlapping 21 different copies of the individual N and C-terminal DHQS domains further reveals a series of pivot points about which these movements occur and illustrates the way in which widely separated secondary structure elements are mechanically inter-linked to form "composite elements", which propagate structural changes across large distances. This analysis has provided insight into the basis of DHQS ligand-initiated domain closure and gives rise to the proposal of an ordered sequence of events involving substrate binding, and local rearrangements within the active site that are propagated to the hinge regions, leading to closure of the active-site cleft.

PubMed: 12614613
DOI: 10.1016/S0022-2836(03)00086-X

Experimental method

X-RAY DIFFRACTION (2.7 Å)