1NUS

CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC NMN/NaMN ADENYLYLTRANSFERASE COMPLEXED WITH ATP ANALOG AND NMN

1NUS の概要

• エントリーDOI: 10.2210/pdb1nus/pdb
• 関連するPDBエントリー: 1NUP 1NUQ 1NUR 1NUT 1NUU
• 分子名称: FKSG76, SULFATE ION, BETA-NICOTINAMIDE RIBOSE MONOPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: nad biosynthesis, mitochondria, pyridine adenylyltransferase, enzyme catalysis, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion: Q96T66
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58666.53

構造登録者

Zhang, X.,Kurnasov, O.V.,Karthikeyan, S.,Grishin, N.V.,Osterman, A.L.,Zhang, H. (登録日: 2003-02-01, 公開日: 2003-06-03, 最終更新日: 2023-08-16)

主引用文献

ZHANG, X.,KURNASOV, O.V.,KARTHIKEYAN, S.,GRISHIN, N.V.,OSTERMAN, A.L.,ZHANG, H.
STRUCTURAL CHARACTERIZATION OF A HUMAN CYTOSOLIC NMN/NaMN ADENYLYLTRANSFERASE AND IMPLICATION IN HUMAN NAD BIOSYNTHESIS
J.Biol.Chem., 278:13503-13511, 2003

PubMed Abstract: Pyridine dinucleotides (NAD and NADP) are ubiquitous cofactors involved in hundreds of redox reactions essential for the energy transduction and metabolism in all living cells. In addition, NAD also serves as a substrate for ADP-ribosylation of a number of nuclear proteins, for silent information regulator 2 (Sir2)-like histone deacetylase that is involved in gene silencing regulation, and for cyclic ADP ribose (cADPR)-dependent Ca(2+) signaling. Pyridine nucleotide adenylyltransferase (PNAT) is an indispensable central enzyme in the NAD biosynthesis pathways catalyzing the condensation of pyridine mononucleotide (NMN or NaMN) with the AMP moiety of ATP to form NAD (or NaAD). Here we report the identification and structural characterization of a novel human PNAT (hsPNAT-3) that is located in the cytoplasm and mitochondria. Its subcellular localization and tissue distribution are distinct from the previously identified human nuclear PNAT-1 and PNAT-2. Detailed structural analysis of PNAT-3 in its apo form and in complex with its substrate(s) or product revealed the catalytic mechanism of the enzyme. The characterization of the cytosolic human PNAT-3 provided compelling evidence that the final steps of NAD biosynthesis pathways may exist in mammalian cytoplasm and mitochondria, potentially contributing to their NAD/NADP pool.

PubMed: 12574164
DOI: 10.1074/jbc.M300073200

実験手法

X-RAY DIFFRACTION (2.2 Å)