1NTS

5'(dCCPUPCPCPUPUP)3':3'(rAGGAGGAAA)5', where P=propynyl

1NTS の概要

• エントリーDOI: 10.2210/pdb1nts/pdb
• 関連するPDBエントリー: 1NTQ 1NTT
• NMR情報: BMRB: 5776
• 分子名称: 5'-R(*AP*AP*AP*GP*GP*AP*GP*GP*A)-3', 5'-D(*(5PC)P*(5PC)P*(PDU)P*(5PC)P*(5PC)P*(PDU)P*(PDU))-3' (2 entities in total)
• 機能のキーワード: dna, rna, hybrid, propynyl, rna-dna complex, rna/dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5230.50

構造登録者

Znosko, B.M.,Barnes III, T.W.,Krugh, T.R.,Turner, D.H. (登録日: 2003-01-30, 公開日: 2003-06-10, 最終更新日: 2024-05-01)

主引用文献

Znosko, B.M.,Barnes III, T.W.,Krugh, T.R.,Turner, D.H.
NMR Studies of DNA Single Strands and DNA:RNA Hybrids With and Without 1-Propynylation at C5 of Oligopyrimidines
J.Am.Chem.Soc., 125:6090-6097, 2003

PubMed Abstract: The 1-propynylation at C5 of consecutive pyrimidines in DNA can enhance DNA:RNA hybrid stability at 37 degrees C by over 1 kcal/mol of substitution [Barnes, T. W., III; Turner, D. H. J. Am. Chem. Soc.2001, 123, 4107-4118]. To provide information on the structural consequences of propynylation, two-dimensional NMR spectroscopy was used to study the structures of several oligonucleotides. Intraresidue nuclear Overhauser effect spectroscopy cross peaks were observed at 30 degrees C and a 200 ms mixing time in the H6-H1' region for 5'(dC(P)C(P)U(P)C(P)C(P)U(P)U(P)) (ssPrODN) but not for 5'(dCCUCCUU) (ssODN), suggesting preorganization of the propynylated single strand. NMR structures of the duplexes 5'(dC(P)C(P)U(P)C(P)C(P)U(P)U(P))3':3'(rGAGGAGGAAAU)5' (PrODN:RNA), 5'(dCC(P)U(P)C(P)C(P)U(P)U(P))3':3'(rGAGGAGGAAAU)5' (sPrODN1:RNA), and 5'(dCCUCCUU)3':3'(rGAGGAGGAAAU)5' (ODN:RNA) indicate that their global structures are almost identical. The NMR data, however, suggest that the 5'-end of sPrODN1:RNA is more dynamic than that of PrODN:RNA. In the propynylated duplexes, the propyne group stacks on the aromatic ring of the 5'-base and extends into the major groove. The results suggest that the increased stability of the propynylated duplexes is caused by preorganization of the propynylated single strand and different interactions in the double strand. The propynyl group provides volume exclusion, enhanced stacking, and possibly different solvation.

PubMed: 12785839
DOI: 10.1021/ja021285d

実験手法

SOLUTION NMR