1NTN

THE CRYSTAL STRUCTURE OF NEUROTOXIN-I FROM NAJA NAJA OXIANA AT 1.9 ANGSTROMS RESOLUTION

1NTN の概要

• エントリーDOI: 10.2210/pdb1ntn/pdb
• 分子名称: NEUROTOXIN I (2 entities in total)
• 機能のキーワード: postsynaptic neurotoxin
• 由来する生物種: Naja oxiana (Central Asian cobra)
• 細胞内の位置: Secreted : P01382
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7942.11

構造登録者

Mikhailov, A.M.,Nickitenko, A.V.,Vainshtein, B.K.,Betzel, C.,Wilson, K. (登録日: 1994-09-26, 公開日: 1995-05-08, 最終更新日: 2024-10-30)

主引用文献

Nickitenko, A.V.,Michailov, A.M.,Betzel, C.,Wilson, K.S.
Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 A resolution.
Febs Lett., 320:111-117, 1993

PubMed Abstract: Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 A, b = 75.6 A, c = 35.9 A, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 A have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 A resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.

PubMed: 8458425
DOI: 10.1016/0014-5793(93)80073-4

実験手法

X-RAY DIFFRACTION (1.9 Å)