1NSH

Solution Structure of Rabbit apo-S100A11 (19 models)

1NSH の概要

• エントリーDOI: 10.2210/pdb1nsh/pdb
• NMR情報: BMRB: 5189
• 分子名称: Calgizzarin (1 entity in total)
• 機能のキーワード: calcium-binding protein, ef hand, helix-loop-helix, s100, annexin, metal binding protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Cytoplasm: P24480
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22625.88

構造登録者

Dempsey, A.C.,Walsh, M.P.,Shaw, G.S. (登録日: 2003-01-27, 公開日: 2003-07-15, 最終更新日: 2024-05-22)

主引用文献

Dempsey, A.C.,Walsh, M.P.,Shaw, G.S.
Unmasking the Annexin I Interaction from the structure of apo-S100A11
Structure, 11:887-897, 2003

PubMed Abstract: S100A11 is a homodimeric EF-hand calcium binding protein that undergoes a calcium-induced conformational change and interacts with the phospholipid binding protein annexin I to coordinate membrane association. In this work, the solution structure of apo-S100A11 has been determined by NMR spectroscopy to uncover the details of its calcium-induced structural change. Apo-S100A11 forms a tight globular structure having a near antiparallel orientation of helices III and IV in calcium binding site II. Further, helices I and IV, and I and I', form a more closed arrangement than observed in other apo-S100 proteins. This helix arrangement in apo-S100A11 partially buries residues in helices I (P3, E11, A15), III (V55, R58, M59), and IV (A86, C87, S90) and the linker (A45, F46), which are required for interaction with annexin I in the calcium-bound state. In apo-S100A11, this results in a "masked" binding surface that prevents annexin I binding but is uncovered upon calcium binding.

PubMed: 12842051
DOI: 10.1016/S0969-2126(03)00126-6

実験手法

SOLUTION NMR