1NR0

Two Seven-Bladed Beta-Propeller Domains Revealed By The Structure Of A C. elegans Homologue Of Yeast Actin Interacting Protein 1 (AIP1).

1NR0 の概要

• エントリーDOI: 10.2210/pdb1nr0/pdb
• 分子名称: Actin interacting protein 1, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: beta propeller, wd40 repeat, actin interacting protein, adf, cofilin, structural genomics, psi, protein structure initiative, new york sgx research center for structural genomics, nysgxrc, structural protein
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65447.82

構造登録者

Vorobiev, S.M.,Mohri, K.,Ono, S.,Almo, S.C.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2003-01-23, 公開日: 2003-07-01, 最終更新日: 2024-02-14)

主引用文献

Mohri, K.,Vorobiev, S.M.,Fedorov, A.A.,Almo, S.C.,Ono, S.
Identification of Functional Residues on Caenorhabditis elegans Actin-interacting Protein 1 (UNC-78) for Disassembly of Actin Depolymerizing Factor/Cofilin-bound Actin Filaments
J.Biol.Chem., 279:31697-31707, 2004

PubMed Abstract: Actin-interacting protein 1 (AIP1) is a WD40 repeat protein that enhances actin filament disassembly in the presence of actin-depolymerizing factor (ADF)/cofilin. AIP1 also caps the barbed end of ADF/cofilin-bound actin filament. However, the mechanism by which AIP1 interacts with ADF/cofilin and actin is not clearly understood. We determined the crystal structure of Caenorhabditis elegans AIP1 (UNC-78), which revealed 14 WD40 modules arranged in two seven-bladed beta-propeller domains. The structure allowed for the mapping of conserved surface residues, and mutagenesis studies identified five residues that affected the ADF/cofilin-dependent actin filament disassembly activity. Mutations of these residues, which reside in blades 3 and 4 in the N-terminal propeller domain, had significant effects on the disassembly activity but did not alter the barbed end capping activity. These data support a model in which this conserved surface of AIP1 plays a direct role in enhancing fragmentation/depolymerization of ADF/cofilin-bound actin filaments but not in barbed end capping.

PubMed: 15150269
DOI: 10.1074/jbc.M403351200

実験手法

X-RAY DIFFRACTION (1.7 Å)