1NO8

SOLUTION STRUCTURE OF THE NUCLEAR FACTOR ALY RBD DOMAIN

1NO8 の概要

• エントリーDOI: 10.2210/pdb1no8/pdb
• NMR情報: BMRB: 5764
• 分子名称: ALY (1 entity in total)
• 機能のキーワード: rbd, aly, ref1-i, bef, mrna export factor, rna binding protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: O08583
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11508.90

構造登録者

Perez-Alvarado, G.C.,Martinez-Yamout, M.,Allen, M.M.,Grosschedl, R.,Dyson, H.J.,Wright, P.E. (登録日: 2003-01-15, 公開日: 2003-08-12, 最終更新日: 2024-05-22)

主引用文献

Perez-Alvarado, G.C.,Martinez-Yamout, M.,Allen, M.M.,Grosschedl, R.,Dyson, H.J.,Wright, P.E.
Structure of the Nuclear Factor ALY: Insights into Post-Transcriptional Regulatory and mRNA Nuclear Export Processes
Biochemistry, 42:7348-7357, 2003

PubMed Abstract: ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with components of a multiprotein postsplicing complex that is deposited 20-24 nucleotides upstream of exon-exon junctions. ALY has a conserved RNA binding domain (RBD) flanked by Gly-Arg rich N-terminal and C-terminal sequences. We determined the solution structure of the RBD homology region in ALY by nuclear magnetic resonance methods. The RBD motif in ALY has a characteristic beta(1)alpha(1)beta(2)-beta(3)alpha(2)beta(4) fold, consisting of a beta sheet composed of four antiparallel beta strands and two alpha helices that pack on one face of the beta sheet. As in other RBD structures, the beta sheet has an exposed face with hydrophobic and charged residues that could modulate interactions with other molecules. The loop that connects beta strands 2 and 3 is in intermediate motion in the NMR time scale, which is also characteristic of other RBDs. This loop presents side chains close to the exposed surface of the beta sheet and is a primary candidate site for intermolecular interactions. The structure of the conserved RBD of ALY provides insight into the nature of interactions involving this multifunctional protein.

PubMed: 12809490
DOI: 10.1021/bi034062o

実験手法

SOLUTION NMR