1NN7

Crystal Structure Of The Tetramerization Domain Of The Shal Voltage-Gated Potassium Channel

Summary for 1NN7

• Entry DOI: 10.2210/pdb1nn7/pdb
• Related: 1A68 3KVT
• Descriptor: potassium channel Kv4.2, ZINC ION (3 entities in total)
• Functional Keywords: t1, teteramerization domain, voltage gated potassium channel, kv4.2, shal, membrane protein
• Biological source: Rattus norvegicus (Norway rat)
• Cellular location: Cell membrane ; Multi- pass membrane protein : Q63881
• Total number of polymer chains: 1
• Total formula weight: 12850.56

Authors

Zhou, W.,Choe, S. (deposition date: 2003-01-13, release date: 2003-07-15, Last modification date: 2023-08-16)

Primary citation

Nanao, M.H.,Zhou, W.,Pfaffinger, P.J.,Choe, S.
Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal)
Proc.Natl.Acad.Sci.USA, 100:8670-8675, 2003

PubMed Abstract: We have developed a semiempirical algorithm called Family Values (FamVal), which identifies residues that encode functional specificity in a protein sequence. Given a multiple sequence alignment (MSA) grouped into functionally distinct subfamilies, FamVal calculates a specificity score for each subfamily at every amino acid position of an MSA. This algorithm was used to predict specificity-encoding positions within the tetramerization assembly (T1) domain of voltage-gated potassium (Kv) channel subfamilies Kv3 and Kv4. The importance of one such position (Arg to Ala at MSA position 93) was confirmed by in vitro pull-down assays. The structural basis of this assembly discrimination was elucidated by determining the crystal structure of the Kv4 T1 domain and comparing it to the Kv3 T1 domain.

PubMed: 12835418
DOI: 10.1073/pnas.1432840100

Experimental method

X-RAY DIFFRACTION (2.1 Å)