1NN7

Crystal Structure Of The Tetramerization Domain Of The Shal Voltage-Gated Potassium Channel

1NN7 の概要

• エントリーDOI: 10.2210/pdb1nn7/pdb
• 関連するPDBエントリー: 1A68 3KVT
• 分子名称: potassium channel Kv4.2, ZINC ION (3 entities in total)
• 機能のキーワード: t1, teteramerization domain, voltage gated potassium channel, kv4.2, shal, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cell membrane ; Multi- pass membrane protein : Q63881
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12850.56

構造登録者

Zhou, W.,Choe, S. (登録日: 2003-01-13, 公開日: 2003-07-15, 最終更新日: 2023-08-16)

主引用文献

Nanao, M.H.,Zhou, W.,Pfaffinger, P.J.,Choe, S.
Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal)
Proc.Natl.Acad.Sci.USA, 100:8670-8675, 2003

PubMed Abstract: We have developed a semiempirical algorithm called Family Values (FamVal), which identifies residues that encode functional specificity in a protein sequence. Given a multiple sequence alignment (MSA) grouped into functionally distinct subfamilies, FamVal calculates a specificity score for each subfamily at every amino acid position of an MSA. This algorithm was used to predict specificity-encoding positions within the tetramerization assembly (T1) domain of voltage-gated potassium (Kv) channel subfamilies Kv3 and Kv4. The importance of one such position (Arg to Ala at MSA position 93) was confirmed by in vitro pull-down assays. The structural basis of this assembly discrimination was elucidated by determining the crystal structure of the Kv4 T1 domain and comparing it to the Kv3 T1 domain.

PubMed: 12835418
DOI: 10.1073/pnas.1432840100

実験手法

X-RAY DIFFRACTION (2.1 Å)