1NM8
Structure of Human Carnitine Acetyltransferase: Molecular Basis for Fatty Acyl Transfer
1NM8 の概要
エントリーDOI | 10.2210/pdb1nm8/pdb |
分子名称 | Carnitine O-acetyltransferase (2 entities in total) |
機能のキーワード | two equally sized domains, anti-parallel beta-strand, transferase |
由来する生物種 | Homo sapiens (human) |
細胞内の位置 | Endoplasmic reticulum (Potential). Isoform 1: Mitochondrion (Potential). Isoform 2: Peroxisome (Potential): P43155 |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 69902.87 |
構造登録者 | Wu, D.,Govindasamy, L.,Lian, W.,Gu, Y.,Kukar, T.,Agbandje-McKenna, M.,McKenna, R. (登録日: 2003-01-09, 公開日: 2003-03-11, 最終更新日: 2024-02-14) |
主引用文献 | Wu, D.,Govindasamy, L.,Lian, W.,Gu, Y.,Kukar, T.,Agbandje-McKenna, M.,McKenna, R. Structure of Human Carnitine Acetyltransferase. Molecular Basis for Fatty Acyl Transfer J.Biol.Chem., 278:13159-13165, 2003 Cited by PubMed Abstract: Carnitine acyltransferases are a family of ubiquitous enzymes that play a pivotal role in cellular energy metabolism. We report here the x-ray structure of human carnitine acetyltransferase to a 1.6-A resolution. This structure reveals a monomeric protein of two equally sized alpha/beta domains. Each domain is shown to have a partially similar fold to other known but oligomeric enzymes that are also involved in group-transfer reactions. The unique monomeric arrangement of the two domains constitutes a central narrow active site tunnel, indicating a likely universal feature for all members of the carnitine acyltransferase family. Superimposition of the substrate complex of a related protein, dihydrolipoyl trans-acetylase, reveals that both substrates localize to the active site tunnel of human carnitine acetyltransferase, suggesting the location of the ligand binding sites for carnitine and coenzyme A. Most significantly, this structure provides critical insights into the molecular basis for fatty acyl chain transfer and a possible common mechanism among a wide range of acyltransferases utilizing a catalytic dyad. PubMed: 12562770DOI: 10.1074/jbc.M212356200 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.6 Å) |
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