1NM7

Solution structure of the ScPex13p SH3 domain

1NM7 の概要

• エントリーDOI: 10.2210/pdb1nm7/pdb
• 関連するPDBエントリー: 1N5Z
• 分子名称: Peroxisomal Membrane Protein PAS20 (1 entity in total)
• 機能のキーワード: yeast, membrane protein, pex5p, pex14p, pex13p, import machine, sh3 domain, protein transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Peroxisome membrane; Single-pass membrane protein: P80667
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8086.21

構造登録者

Pires, J.R.,Hong, X.,Brockmann, C.,Volkmer-Engert, R.,Schneider-Mergener, J.,Oschkinat, H.,Erdmann, R. (登録日: 2003-01-09, 公開日: 2003-03-04, 最終更新日: 2024-05-29)

主引用文献

Pires, J.R.,Hong, X.,Brockmann, C.,Volkmer-Engert, R.,Schneider-Mergener, J.,Oschkinat, H.,Erdmann, R.
The ScPex13p SH3 Domain Exposes Two Distinct Binding Sites for Pex5p and Pex14p
J.Mol.Biol., 326:1427-1435, 2003

PubMed Abstract: Pex13p is an essential component of the peroxisomal protein import machinery and interacts via its C-terminal SH3 domain with the type II SH3-ligand Pex14p and the non-PXXP protein Pex5p. We report the solution structure of the SH3 domain of Pex13p from Saccharomyces cerevisiae and the identification of a novel-binding pocket, which binds a non-PXXP-peptide representing the binding site of Pex5p. Chemical shift assays revealed the binding sites for Pex5p and Pex14p ligand peptides to be distinct and spatially separated. Competition assays demonstrated that the two ligand peptides can bind simultaneously to the SH3 domain.

PubMed: 12595255
DOI: 10.1016/S0022-2836(03)00039-1

実験手法

SOLUTION NMR