1NHV

Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor

Summary for 1NHV

DescriptorHEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE, (2S)-2-[(5-BENZOFURAN-2-YL-THIOPHEN-2-YLMETHYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-3-PHENYL-PROPIONIC ACID (2 entities in total)
Functional Keywordshepatitis c; rna polymerase; enzyme inhibition, transferase
Biological sourceHepatitis C virus subtype 1b
Cellular locationCore protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein. Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane ; Peripheral membrane protein . RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein  P26663
Total number of polymer chains2
Total molecular weight129624.13
Authors
Wang, M.,Ng, K.K.S.,Cherney, M.M.,Chan, L.,Yannopoulos, C.G.,Bedard, J.,Morin, N.,Nguyen-Ba, N.,Alaoui-Ismaili, M.H.,Bethell, R.C.,James, M.N.G. (deposition date: 2002-12-19, release date: 2003-03-18, Last modification date: 2017-10-11)
Primary citation
Wang, M.,Ng, K.K.S.,Cherney, M.M.,Chan, L.,Yannopoulos, C.G.,Bedard, J.,Morin, N.,Nguyen-Ba, N.,Alaoui-Ismaili, M.H.,Bethell, R.C.,James, M.N.G.
Non-Nucleoside Analogue Inhibitors Bind to an Allosteric Site on HCV NS5B Polymerase: Crystal Structures and Mechanism of Inhibition
J.Biol.Chem., 278:9489-9495, 2003
PubMed: 12509436 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M209397200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.9 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers220.7%3.5%6.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report