1NGT

The Role of Minor Groove Functional Groups in DNA Hydration

Summary for 1NGT

• Entry DOI: 10.2210/pdb1ngt/pdb
• Descriptor: 5'-D(*CP*GP*CP*GP*AP*AP*(MTR)P*TP*CP*GP*CP*G)-3', MAGNESIUM ION (3 entities in total)
• Functional Keywords: spine of hydration, dna conformation, modified base, dna
• Total number of polymer chains: 2
• Total formula weight: 7317.12

Authors

Woods, K.K.,Lan, T.,McLaughlin, L.W.,Williams, L.D. (deposition date: 2002-12-17, release date: 2003-03-04, Last modification date: 2024-02-14)

Primary citation

Woods, K.K.,Lan, T.,McLaughlin, L.W.,Williams, L.D.
The Role of Minor Groove Functional Groups in DNA Hydration
Nucleic Acids Res., 31:1536-1540, 2003

PubMed Abstract: Here we describe the crystal structure of modified [d(CGCGAATTCGCG)]2 refined to 2.04 A. The modification, which affects only the two thymines at the central ApT step, involves isosteric removal of the 2-keto oxygen atoms and substitution of the N1 nitrogen with carbon. The crystal structure reveals the ability of this modified thymine to effectively base pair with adenine in [d(CGCGAAtTCGCG)]2. The structure also suggests that the minor groove 'spine of hydration' is destabilized but essentially intact.

PubMed: 12595562
DOI: 10.1093/nar/gkg240

Experimental method

X-RAY DIFFRACTION (2.04 Å)