1NEY

Triosephosphate Isomerase in Complex with DHAP

1NEY の概要

• エントリーDOI: 10.2210/pdb1ney/pdb
• 関連するPDBエントリー: 1I45 1NF0
• 分子名称: triosephosphate isomerase, 1,3-DIHYDROXYACETONEPHOSPHATE (3 entities in total)
• 機能のキーワード: yeast, triosephosphate isomerase, dhap, dihydroxyacetone phosphate, michaelis complex, isomerase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53644.41

構造登録者

Jogl, G.,Rozovsky, S.,McDermott, A.E.,Tong, L. (登録日: 2002-12-12, 公開日: 2003-01-07, 最終更新日: 2023-08-16)

主引用文献

Jogl, G.,Rozovsky, S.,McDermott, A.E.,Tong, L.
Optimal alignment for enzymatic proton transfer: Structure of the Michaelis complex of triosephosphate isomerase at 1.2-A resolution.
Proc.Natl.Acad.Sci.USA, 100:50-55, 2003

PubMed Abstract: In enzyme catalysis, where exquisitely positioned functionality is the sine qua non, atomic coordinates for a Michaelis complex can provide powerful insights into activation of the substrate. We focus here on the initial proton transfer of the isomerization reaction catalyzed by triosephosphate isomerase and present the crystal structure of its Michaelis complex with the substrate dihydroxyacetone phosphate at near-atomic resolution. The active site is highly compact, with unusually short and bifurcated hydrogen bonds for both catalytic Glu-165 and His-95 residues. The carboxylate oxygen of the catalytic base Glu-165 is positioned in an unprecedented close interaction with the ketone and the alpha-hydroxy carbons of the substrate (C em leader O approximately 3.0 A), which is optimal for the proton transfer involving these centers. The electrophile that polarizes the substrate, His-95, has close contacts to the substrate's O1 and O2 (N em leader O < or = 3.0 and 2.6 A, respectively). The substrate is conformationally relaxed in the Michaelis complex: the phosphate group is out of the plane of the ketone group, and the hydroxy and ketone oxygen atoms are not in the cisoid configuration. The epsilon ammonium group of the electrophilic Lys-12 is within hydrogen-bonding distance of the substrate's ketone oxygen, the bridging oxygen, and a terminal phosphate's oxygen, suggesting a role for this residue in both catalysis and in controlling the flexibility of active-site loop.

PubMed: 12509510
DOI: 10.1073/pnas.0233793100

実験手法

X-RAY DIFFRACTION (1.2 Å)