1NEE

Structure of archaeal translation factor aIF2beta from Methanobacterium thermoautrophicum

1NEE の概要

• エントリーDOI: 10.2210/pdb1nee/pdb
• NMR情報: BMRB: 5312
• 分子名称: Probable translation initiation factor 2 beta subunit, ZINC ION (2 entities in total)
• 機能のキーワード: two domain protein, mixed alpha-beta structure, zinc finger, translation
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15958.67

構造登録者

Gutierrez, P.,Trempe, J.F.,Siddiqui, N.,Arrowsmith, C.,Gehring, K. (登録日: 2002-12-11, 公開日: 2004-03-09, 最終更新日: 2024-05-22)

主引用文献

Gutierrez, P.,Osborne, M.J.,Siddiqui, N.,Trempe, J.F.,Arrowsmith, C.,Gehring, K.
Structure of the archaeal translation initiation factor aIF2beta from Methanobacterium thermoautotrophicum: Implications for translation initiation.
Protein Sci., 13:659-667, 2004

PubMed Abstract: aIF2 beta is the archaeal homolog of eIF2 beta, a member of the eIF2 heterotrimeric complex, implicated in the delivery of Met-tRNA(i)(Met) to the 40S ribosomal subunit. We have determined the solution structure of the intact beta-subunit of aIF2 from Methanobacterium thermoautotrophicum. aIF2 beta is composed of an unfolded N terminus, a mixed alpha/beta core domain and a C-terminal zinc finger. NMR data shows the two folded domains display restricted mobility with respect to each other. Analysis of the aIF2 gamma structure docked to tRNA allowed the identification of a putative binding site for the beta-subunit in the ternary translation complex. Based on structural similarity and biochemical data, a role for the different secondary structure elements is suggested.

PubMed: 14978306
DOI: 10.1110/ps.03506604

実験手法

SOLUTION NMR