1NCV

DETERMINATION CC-CHEMOKINE MCP-3, NMR, 7 STRUCTURES

1NCV の概要

• エントリーDOI: 10.2210/pdb1ncv/pdb
• 分子名称: MONOCYTE CHEMOATTRACTANT PROTEIN 3 (1 entity in total)
• 機能のキーワード: mcp-3, beta-chemokine, cytokine, chemotaxis, heparin-binding, glycoprotein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P80098
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17948.96

構造登録者

Meunier, S.,Bernassau, J.M.,Guillemot, J.C.,Ferrara, P.,Darbon, H. (登録日: 1997-02-05, 公開日: 1997-10-15, 最終更新日: 2024-10-23)

主引用文献

Meunier, S.,Bernassau, J.M.,Guillemot, J.C.,Ferrara, P.,Darbon, H.
Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy.
Biochemistry, 36:4412-4422, 1997

PubMed Abstract: MCP-3 is a beta chemokine consisting of 76 amino acid residues. It has been described to be involved in the activation of all leukocytic cells, activation mediated by the presence of multiple binding sites on the target cells. Its three-dimensional structure has been studied by making use of two-dimensional 1H NMR spectroscopy. MCP-3 exhibits the same monomeric structure as the other chemokines, i.e., a three-stranded antiparallel beta sheet covered on one face by an alpha helix. Although it belongs to the same subfamily as RANTES (Chung et al., 1995; Faitbrother et al., 1994) and hMIP-1beta (Lodi et al., 1994), the MCP-3 dimer is folded like IL-8 with the so-called alphabeta sandwich structural motif. Structural and sequence analysis gives clear indications suggesting that the other MCP chemokines may have the same quaternary structure, contrary to the other beta chemokines.

PubMed: 9109648
DOI: 10.1021/bi9627929

実験手法

SOLUTION NMR