1NBY

Crystal Structure of HyHEL-63 complexed with HEL mutant K96A

1NBY の概要

• エントリーDOI: 10.2210/pdb1nby/pdb
• 関連するPDBエントリー: 1DQJ 1DQM 1DQQ 1NBZ 1NDG 1NDM
• 分子名称: antibody kappa light chain, immunoglobulin gamma 1 chain, Lysozyme C, ... (4 entities in total)
• 機能のキーワード: antibody, lysozyme, mutant, immune system-hydrolase complex, immune system/hydrolase
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Cell membrane; Single-pass membrane protein (Potential): P01865; Secreted: P00698
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60412.95

構造登録者

Mariuzza, R.A.,Li, Y.,Urrutia, M.,Smith-Gill, S.J. (登録日: 2002-12-04, 公開日: 2003-04-01, 最終更新日: 2024-10-30)

主引用文献

Li, Y.,Urrutia, M.,Smith-Gill, S.J.,Mariuzza, R.A.
Dissection of binding interactions in the complex between the anti-lysozyme antibody HyHEL-63 and its antigen
Biochemistry, 42:11-22, 2003

PubMed Abstract: Alanine-scanning mutagenesis, X-ray crystallography, and double mutant cycles were used to characterize the interface between the anti-hen egg white lysozyme (HEL) antibody HyHEL-63 and HEL. Eleven HEL residues in contact with HyHEL-63 in the crystal structure of the antigen-antibody complex, and 10 HyHEL-63 residues in contact with HEL, were individually truncated to alanine in order to determine their relative contributions to complex stabilization. The residues of HEL (Tyr20, Lys96, and Lys97) most important for binding HyHEL-63 (Delta G(mutant) - Delta G(wild type) > 3.0 kcal/mol) form a contiguous patch at the center of the surface contacted by the antibody. Hot spot residues of the antibody (Delta Delta G > 2.0 kcal/mol) are organized in two clusters that juxtapose hot spot residues of HEL, resulting in energetic complementarity across the interface. All energetically critical residues are centrally located, shielded from solvent by peripheral residues that contribute significantly less to the binding free energy. Although HEL hot spot residues Lys96 and Lys97 make similar interactions with antibody in the HyHEL-63/HEL complex, alanine substitution of Lys96 results in a nearly 100-fold greater reduction in affinity than the corresponding mutation in Lys97. To understand the basis for this marked difference, we determined the crystal structures of the HyHEL-63/HEL Lys96Ala and HyHEL-63/HEL Lys97Ala complexes to 1.80 and 1.85 A resolution, respectively. Whereas conformational changes in the proteins and differences in the solvent networks at the mutation sites appear too small to explain the observed affinity difference, superposition of free HEL in different crystal forms onto bound HEL in the wild type and mutant HyHEL-63/HEL complexes reveals that the side-chain conformation of Lys96 is very similar in the various structures, but that the Lys97 side chain displays considerable flexibility. Accordingly, a greater entropic penalty may be associated with quenching the mobility of the Lys97 than the Lys96 side chain upon complex formation, reducing binding. To further dissect the energetics of specific interactions in the HyHEL-63/HEL interface, double mutant cycles were constructed to measure the coupling of 13 amino acid pairs, 11 of which are in direct contact in the crystal structure. A large coupling energy, 3.0 kcal/mol, was found between HEL residue Lys97 and HyHEL-63 residue V(H)Asp32, which form a buried salt bridge surrounded by polar residues of the antigen. Thus, in contrast to protein folding where buried salt bridges are generally destabilizing, salt bridges in protein-protein interfaces, whose residual composition is more hydrophilic than that of protein interiors, may contribute significantly to complex stabilization.

PubMed: 12515535
DOI: 10.1021/bi020589+

実験手法

X-RAY DIFFRACTION (1.8 Å)