1NBL

NMR Structure of Hellethionin D

1NBL の概要

• エントリーDOI: 10.2210/pdb1nbl/pdb
• NMR情報: BMRB: 5670
• 分子名称: Hellethionin D (1 entity in total)
• 機能のキーワード: gamma thionins helleborus, toxin
• 由来する生物種: Helleborus purpurascens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4915.62

構造登録者

Milbradt, A.G.,Kerek, F.,Moroder, L.,Renner, C. (登録日: 2002-12-03, 公開日: 2003-03-11, 最終更新日: 2024-11-20)

主引用文献

Milbradt, A.G.,Kerek, F.,Moroder, L.,Renner, C.
Structural Characterization of Hellethionins from Helleborus purpurascens
Biochemistry, 42:2404-2411, 2003

PubMed Abstract: Thionins are relatively small-sized multiple-cystine peptides that are probably involved in the plant defense against pathogens. As such, these peptides constitute promising candidates for engineered plant resistance in the agricultural industry. More recently, thionins have been proposed as potential immunotoxins in tumor therapy. In the search for pharmacologically active natural products, a new family of thionins was recently discovered in the roots of Helleborus purpurascens that accordingly were termed hellethionins. The structural characterization by NMR of one representative member of this family, i.e., of hellethionin D, clearly reveals that thionins from different sources share a highly conserved overall fold. In fact, the well-defined 3D structure of hellethionin D is very similar to those reported so far for viscotoxins, purothionins, or crambin, although distinct differences could be detected in the C-terminal portion, especially for loop 36-39. These differences may derive from the unusual distribution of charged residues in the C-terminal half of the peptide sequence compared to other thionins and from the uncommon occurrence of four contiguous threonine residues in loop 36-39. As expected, reduction of the disulfide bonds in hellethionin D leads to complete unfolding, but upon oxidative refolding by air oxygen in the presence of glutathione the correct isomer is recovered in high yields, confirming the very robust fold of this class of bioactive cystine peptides.

PubMed: 12600207
DOI: 10.1021/bi020628h

実験手法

SOLUTION NMR