1NBJ

High-resolution solution structure of cycloviolacin O1

1NBJ の概要

• エントリーDOI: 10.2210/pdb1nbj/pdb
• 関連するPDBエントリー: 1DF6
• 分子名称: cycloviolacin O1 (1 entity in total)
• 機能のキーワード: cyclotide, cyclic backbone, cystine knot, cck, plant protein
• 由来する生物種: Viola odorata
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3141.69

構造登録者

Rosengren, K.J.,Daly, N.L.,Plan, M.R.,Waine, C.,Craik, D.J. (登録日: 2002-12-02, 公開日: 2003-03-18, 最終更新日: 2024-10-23)

主引用文献

Rosengren, K.J.,Daly, N.L.,Plan, M.R.,Waine, C.,Craik, D.J.
Twists, Knots, and Rings in Proteins. STRUCTURAL DEFINITION OF THE CYCLOTIDE FRAMEWORK.
J.Biol.Chem., 278:8606-8626, 2003

PubMed Abstract: In recent years an increasing number of miniproteins containing an amide-cyclized backbone have been discovered. The cyclotide family is the largest group of such proteins and is characterized by a circular protein backbone and six conserved cysteine residues linked by disulfide bonds in a tight core of the molecule. These form a cystine knot in which an embedded ring formed by two of the disulfide bonds and the connecting backbone segment is threaded by a third disulfide bond. In the current study we have undertaken high resolution structural analysis of two prototypic cyclotides, kalata B1 and cycloviolacin O1, to define the role of the conserved residues in the sequence. We provide the first comprehensive analysis of the topological features in this unique family of proteins, namely rings (a circular backbone), twists (a cis-peptide bond in the Möbius cyclotides) and knots (a knotted arrangement of the disulfide bonds).

PubMed: 12482868
DOI: 10.1074/jbc.M211147200

実験手法

SOLUTION NMR