1NB2

Crystal Structure of Nucleoside Diphosphate Kinase from Bacillus Halodenitrificans

1NB2 の概要

• エントリーDOI: 10.2210/pdb1nb2/pdb
• 分子名称: Nucleoside Diphosphate Kinase (2 entities in total)
• 機能のキーワード: nucleoside diphosphate kinase, bacillus halodenitrifians, transferase
• 由来する生物種: Virgibacillus halodenitrificans
• 細胞内の位置: Cytoplasm (By similarity): Q7SIA9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14883.73

構造登録者

Chen, C.-J.,Liu, M.-Y.,Chang, W.-C.,Chang, T.,Wang, B.-C.,Le Gall, J. (登録日: 2002-12-02, 公開日: 2003-05-06, 最終更新日: 2024-03-13)

主引用文献

Chen, C.-J.,Liu, M.-Y.,Chang, T.,Chang, W.-C.,Wang, B.-C.,Le Gall, J.
Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans: coexpression of its activity with a Mn-superoxide dismutase.
J.Struct.Biol., 142:247-255, 2003

PubMed Abstract: We found that when grown under anaerobic conditions the moderate halophile, gram-positive bacterium Bacillus halodenitrificans (ATCC 49067) synthesizes large amounts of a polypeptide complex that contains a heme center capable of reversibly bind nitric oxide. This complex, when exposed to air, dissociates and reassociates into two active components, a Mn-containing superoxide dismutase (SOD) and a nucleoside diphosphate kinase (BhNDK). The crystal structure of this latter enzyme has been determined at 2.2A resolution using molecular replacement method, based on the crystal structure of Drosophila melanogaster NDK. The model contains 149 residues of a total 150 residues and 34 water molecules. BhNDK consists of a four-stranded antiparallel beta-sheet, whose surfaces are partially covered by six alpha-helices, and its overall and active site structures are similar to those of homologous enzymes. However, the hexameric packing of BhNDK shows that this enzyme is different from both eukaryotic and gram-negative bacteria. The need for the bacterium to presynthesize both SOD and NDK precursors which are activated during the anaerobic-aerobic transition is discussed.

PubMed: 12713952
DOI: 10.1016/S1047-8477(03)00014-5

実験手法

X-RAY DIFFRACTION (2.2 Å)