1NA7

Crystal structure of the catalytic subunit of human protein kinase CK2

1NA7 の概要

• エントリーDOI: 10.2210/pdb1na7/pdb
• 関連するPDBエントリー: 1A6O 1F0Q 1M2Q 1M2R
• 分子名称: Casein kinase II, alpha chain (2 entities in total)
• 機能のキーワード: ck2, protein kinase, microcrystals, thin-film nanotechnology, microfocus esrf, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39333.89

構造登録者

Pechkova, E.,Zanotti, G.,Nicolini, C. (登録日: 2002-11-27, 公開日: 2003-08-26, 最終更新日: 2023-08-16)

主引用文献

Pechkova, E.,Zanotti, G.,Nicolini, C.
Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2.
Acta Crystallogr.,Sect.D, 59:2133-2139, 2003

PubMed Abstract: The three-dimensional crystal structure of the triple-point mutant of the catalytic subunit of human protein kinase CK2alpha has been determined at 2.4 A resolution. Microcrystals of mutant CK2 catalytic subunit were obtained by a protein-crystallization method based on thin-film nanotechnology. These microcrystals (of about 20 micro m in diameter) were used for diffraction data collection by means of the microfocus beamline at the ESRF synchrotron. A comparison between the human protein kinase CK2alpha and the corresponding enzyme from a lower organism (Zea mays) is made.

PubMed: 14646071
DOI: 10.1107/S0907444903018900

実験手法

X-RAY DIFFRACTION (2.4 Å)