1N9J

Solution Structure of the 3D domain swapped dimer of Stefin A

1N9J の概要

• エントリーDOI: 10.2210/pdb1n9j/pdb
• 関連するPDBエントリー: 1DVC
• 分子名称: Cystatin A (1 entity in total)
• 機能のキーワード: domain swapped, stefin a, cystatins, amyloid, hydrolase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P01040
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22040.93

構造登録者

Staniforth, R.A.,Giannini, S.,Higgins, L.D.,Conroy, M.J.,Hounslow, A.M.,Jerala, R.,Craven, C.J.,Waltho, J.P. (登録日: 2002-11-25, 公開日: 2003-02-25, 最終更新日: 2024-05-22)

主引用文献

Staniforth, R.A.,Giannini, S.,Higgins, L.D.,Conroy, M.J.,Hounslow, A.M.,Jerala, R.,Craven, C.J.,Waltho, J.P.
Three-dimensional domain swapping in the folded and molten-globule states of cystatins, an amyloid-forming structural superfamily
Embo J., 20:4774-4781, 2001

PubMed Abstract: Cystatins, an amyloid-forming structural superfamily, form highly stable, domain-swapped dimers at physiological protein concentrations. In chicken cystatin, the active monomer is a kinetic trap en route to dimerization, and any changes in solution conditions or mutations that destabilize the folded state shorten the lifetime of the monomeric form. In such circumstances, amyloidogenesis will start from conditions where a domain-swapped dimer is the most prevalent species. Domain swapping occurs by a rearrangement of loop I, generating the new intermonomer interface between strands 2 and 3. The transition state for dimerization has a high level of hydrophobic group exposure, indicating that gross conformational perturbation is required for domain swapping to occur. Dimerization also occurs when chicken cystatin is in its reduced, molten-globule state, implying that the organization of secondary structure in this state mirrors that in the folded state and that domain swapping is not limited to the folded states of proteins. Although the interface between cystatin-fold units is poorly defined for cystatin A, the dimers are the appropriate size to account for the electron-dense regions in amyloid protofilaments.

PubMed: 11532941
DOI: 10.1093/emboj/20.17.4774

実験手法

SOLUTION NMR