1N7A

RIP-Radiation-damage Induced Phasing

Summary for 1N7A

• Entry DOI: 10.2210/pdb1n7a/pdb
• Related: 1N6X 1N6Y 1N7B
• Descriptor: RNA/DNA (5'-R(*U)-D(P*(BGM))-R(P*AP*GP*GP*U)-3'), POTASSIUM ION, SPERMINE, ... (4 entities in total)
• Functional Keywords: dna, rna
• Total number of polymer chains: 8
• Total formula weight: 16717.31

Authors

Ravelli, R.B.G.,Leiros, H.-K.S.,Pan, B.,Caffrey, M.,McSweeney, S. (deposition date: 2002-11-13, release date: 2003-03-04, Last modification date: 2024-02-14)

Primary citation

Ravelli, R.B.G.,Leiros, H.-K.S.,Pan, B.,Caffrey, M.,McSweeney, S.
Specific Radiation-Damage Can Be Used To Solve Macromolecular Crystal Structures
Structure, 11:217-224, 2003

PubMed Abstract: The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.

PubMed: 12575941
DOI: 10.1016/S0969-2126(03)00006-6

Experimental method

X-RAY DIFFRACTION (1.2 Å)