1N6Y

RIP-phasing on Bovine Trypsin

1N6Y の概要

• エントリーDOI: 10.2210/pdb1n6y/pdb
• 関連するPDBエントリー: 1N6X 1N7A 1N7B
• 分子名称: Trypsinogen, cationic, SULFATE ION, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted, extracellular space: P00760
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23755.74

構造登録者

Ravelli, R.B.G.,Leiros, H.-K.S.,Pan, B.,Caffrey, M.,McSweeney, S. (登録日: 2002-11-12, 公開日: 2003-03-04, 最終更新日: 2024-11-13)

主引用文献

Ravelli, R.B.G.,Leiros, H.-K.S.,Pan, B.,Caffrey, M.,McSweeney, S.
Specific Radiation-Damage Can Be Used To Solve Macromolecular Crystal Structures
Structure, 11:217-224, 2003

PubMed Abstract: The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.

PubMed: 12575941
DOI: 10.1016/S0969-2126(03)00006-6

実験手法

X-RAY DIFFRACTION (1.4 Å)