1N4N

Structure of the Plant Defensin PhD1 from Petunia Hybrida

1N4N の概要

• エントリーDOI: 10.2210/pdb1n4n/pdb
• NMR情報: BMRB: 5612
• 分子名称: floral defensin-like protein 1 (1 entity in total)
• 機能のキーワード: cysteine-stabilised alpha-beta motif, fifth disulfide bond, plant protein
• 由来する生物種: Petunia x hybrida
• 細胞内の位置: Secreted (By similarity): Q8H6Q1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5222.34

構造登録者

Janssen, B.J.C.,Schirra, H.J.,Lay, F.T.,Anderson, M.A.,Craik, D.J. (登録日: 2002-11-01, 公開日: 2003-11-01, 最終更新日: 2024-10-16)

主引用文献

Janssen, B.J.C.,Schirra, H.J.,Lay, F.T.,Anderson, M.A.,Craik, D.J.
Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds
Biochemistry, 42:8214-8222, 2003

PubMed Abstract: The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose structure has been determined. PhD1 has the fold of the cysteine-stabilized alphabeta motif, consisting of an alpha-helix and a triple-stranded antiparallel beta-sheet, except that it contains a fifth disulfide bond from the first loop to the alpha-helix. The additional disulfide bond is accommodated in PhD1 without any alteration of its tertiary structure with respect to other plant defensins. Comparison of its structure with those of classic, four-disulfide defensins has allowed us to identify a previously unrecognized hydrogen bond network that is integral to structure stabilization in the family.

PubMed: 12846570
DOI: 10.1021/bi034379o

実験手法

SOLUTION NMR