1N4E

Crystal Structure of a DNA Decamer Containing a Thymine-dimer

1N4E の概要

• エントリーDOI: 10.2210/pdb1n4e/pdb
• 分子名称: 5'-D(*GP*CP*TP*TP*AP*AP*TP*TP*CP*G)-3', 5'-D(*CP*GP*AP*AP*TP*TP*AP*AP*GP*C)-3' (3 entities in total)
• 機能のキーワード: uv-damaged dna, thymine dimer, dna decamer, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12176.07

構造登録者

Park, H.,Zhang, K.,Ren, Y.,Nadji, S.,Sinha, N.,Taylor, J.-S.,Kang, C. (登録日: 2002-10-30, 公開日: 2003-02-04, 最終更新日: 2024-02-14)

主引用文献

Park, H.,Zhang, K.,Ren, Y.,Nadji, S.,Sinha, N.,Taylor, J.S.,Kang, C.
Crystal structure of a DNA decamer containing a cis-syn thymine dimer.
Proc.Natl.Acad.Sci.USA, 99:15965-15970, 2002

PubMed Abstract: It is well known that exposure to UV induces DNA damage, which is the first step in mutagenesis and a major cause of skin cancer. Among a variety of photoproducts, cyclobutane-type pyrimidine photodimers (CPD) are the most abundant primary lesion. Despite its biological importance, the precise relationship between the structure and properties of DNA containing CPD has remained to be elucidated. Here, we report the free (unbound) crystal structure of duplex DNA containing a CPD lesion at a resolution of 2.0 A. Our crystal structure shows that the overall helical axis bends approximately 30 degrees toward the major groove and unwinds approximately 9 degrees, in remarkable agreement with some previous theoretical and experimental studies. There are also significant differences in local structure compared with standard B-DNA, including pinching of the minor groove at the 3' side of the CPD lesion, a severe change of the base pair parameter in the 5' side, and serious widening of both minor and major groves both 3' and 5' of the CPD. Overall, the structure of the damaged DNA differs from undamaged DNA to an extent that DNA repair proteins may recognize this conformation, and the various components of the replicational and transcriptional machinery may be interfered with due to the perturbed local and global structure.

PubMed: 12456887
DOI: 10.1073/pnas.242422699

実験手法

X-RAY DIFFRACTION (2.5 Å)